Collagen, type XVIII, alpha 1, also known as COL18A1,alpha chain of type XVIII collagen. This collagen is one of the multiplexins, extracellular matrix proteins that contain multiple triple-helix domains (collagenous domains) interrupted by non-collagenous domains.The proteolytically produced C-terminal fragment of type XVIII collagen is endostatin, a potent antiangiogenic protein. Mutations in this gene are associated with Knobloch syndrome. The main features of this syndrome involve retinal abnormalities so type XVIII collagen may play an important role in retinal structure and in neural tube closure. Two transcript variants encoding different isoforms have been found for this gene.
Bovine Collagen alpha-1 (XVIII) chian (COL18A1) ELISA Kit employs a two-site sandwich ELISA to quantitate COL18A1 in samples. An antibody specific for COL18A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCOL18A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for COL18A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of COL18A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Bovine Collagen alpha-1 (XVIII) chian (COL18A1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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