Somatotropin is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity.Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed in the pituitary but not in placental tissue as is the case for the other four genes in the growth hormone locus.
Bovine Growth hormone (GH) ELISA Kit employs a two-site sandwich ELISA to quantitate GH1 in samples. An antibody specific for GH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGH1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GH1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GH1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Bovine Growth hormone (GH) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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