PAX6 is the most researched of the Pax genes and appears throughout the literature as a "master control" gene for the development of eyes and other sensory organs, certain neural and epidermal tissues as well as other homologous structures, usually derived from ectodermal tissues. This transcription factor is most famous for its use in the interspecifically induced expression of ectopic eyes and is of medical importance because heterozygous mutants produce a wide spectrum of ocular defects such as Aniridia in humans.PAX6 protein function is highly conserved across bilaterian species, for instance mouse PAX6 can trigger eye development in Drosophila melanogaster.
Bovine Paired box protein Pax-6 (PAX6) ELISA Kit employs a two-site sandwich ELISA to quantitate PAX6 in samples. An antibody specific for PAX6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPAX6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PAX6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PAX6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Bovine Paired box protein Pax-6 (PAX6) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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