Protein geranylgeranyltransferase type I (GGTase-I) transfers a geranylgeranyl group to the cysteine residue of candidate proteins containing a C-terminal CAAX motif in which 'A' is an aliphatic amino acid and 'X' is leucine . The enzyme is composed of a 48-kD alpha subunit (FNTA) and a 43-kD beta subunit, encoded by the PGGT1B gene. The FNTA gene encodes the alpha subunit for both GGTase-I and the related enzyme farnesyltransferase.The sequences from both species encode a 377-amino acid polypeptide with a predicted molecular mass of 42.4 kD (human) and 42.5 kD (rat). Amino acid sequence comparisons suggested that the mammalian beta-subunit protein is the counterpart of the Saccharomyces cerevisiae gene CDC43.
Bovine Geranylgeranyl transferase type-1 subunit beta (PGGT1B) ELISA Kit employs a two-site sandwich ELISA to quantitate PGGT1B in samples. An antibody specific for PGGT1B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPGGT1B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PGGT1B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PGGT1B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Bovine Geranylgeranyl transferase type-1 subunit beta (PGGT1B) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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