DNA polymerase epsilon (POLE) has been implicated in DNA repair and replication. Purified human HeLa POLE consists of a 261-kD catalytic subunit and a 55-kD accessory subunit. Li et al. (1997) cloned the small subunit of human POLE, which they symbolized DPE2. The cDNA predicted a 526-amino acid protein. The gene product has 26% identity to DPB2, a POLE accessory protein in the yeast S. cerevisiae.Li et al. (1997) assigned the DPE2 gene to human chromosome 14 using human-rodent hybrid panels. The gene was then regionally localized to 14q13-q21 by fluorescence in situ hybridization.
Bovine DNA polymerase epsilon subunit 2 (POLE2) ELISA Kit employs a two-site sandwich ELISA to quantitate POLE2 in samples. An antibody specific for POLE2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPOLE2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for POLE2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POLE2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Bovine DNA polymerase epsilon subunit 2 (POLE2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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