POLR2C encodes the third largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a cysteine rich region and exists as a heterodimer with another polymerase subunit, POLR2J. These two subunits form a core subassembly unit of the polymerase. A pseudogene has been identified on chromosome 21.The deduced 275-amino acid human POLR2C protein, termed RPB33 by the authors, shares 45% sequence identity with the yeast homolog RPB3. POLR2C contains a single cysteine-rich region. Northern blot analysis of HeLa cell mRNA detected an approximately 1.8-kb POLR2C doublet.
Bovine DNA-directed RNA polymerase II subunit RPB3 (POLR2C) ELISA Kit employs a two-site sandwich ELISA to quantitate POLR2C in samples. An antibody specific for POLR2C has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPOLR2C present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for POLR2C is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POLR2C bound in the initial step. The color development is stopped and the intensity of the color is measured.
Bovine DNA-directed RNA polymerase II subunit RPB3 (POLR2C) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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