Cortisone is a steroid hormone. Chemically, it is a corticosteroid closely related to corticosterone. It is used to treat a variety of ailments and can be administered intravenously, orally, intraarticularly, or cutaneously. Cortisone suppresses the immune system, thus reducing inflammation and attendant pain and swelling at the site of the injury. However, its effects on the immune system have resulted in various side-effects, particularly in the long-term use of cortisone.17-hydroxycorticosteroid (17-OHCS) is an inactive product formed when the liver and other body tissues break down cortisol, a steroid hormone.17-OHCS is a group of hydroxycorticosteroids bearing a hydroxy group at the 17-position. Urinary excretion of these compounds is used as an index of adrenal function. They are used systemically in the free alcohol form, but with esterification of the hydroxy groups, topical effectiveness is increased.
Bovine Cortisol (COR) ELISA Kit employs a two-site sandwich ELISA to quantitate COR in samples. An antibody specific for COR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCOR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for COR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of COR bound in the initial step. The color development is stopped and the intensity of the color is measured.
Bovine Cortisol (COR) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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