Ferrochelatase (FECH, protoheme ferrolyase) is an enzyme that catalyses the terminal (eighth) step in the biosynthesis of heme, converting protoporphyrin IX into heme. It catalyses reaction: protoporphyrin + Fe++ <=> protoheme + 2 H+. A ferrochelatase enzyme consists of 497 amino acid residues with a m.w. of 55.4 kDa.Ferrochelatase is localized to the mitochondrion where it catalyzes the insertion of the ferrous form of iron into protoporphyrin IX in the heme synthesis pathway. Defects in ferrochelatase are associated with erythropoietic protoporphyria. Two transcript variants encoding different isoforms have been found for this gene.
Bovine Ferrochelatase, mitochondrial (FECH) ELISA Kit employs a two-site sandwich ELISA to quantitate FECH in samples. An antibody specific for FECH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFECH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FECH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FECH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Bovine Ferrochelatase, mitochondrial (FECH) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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