Chorionic somatomammotropin hormone 2 (CSH2) is a member of the somatotropin/prolactin family of hormones and plays an important role in growth control. The gene is located at the growth hormone locus on chromosome 17 along with four other related genes in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. Although the five genes share a remarkably high degree of sequence identity, they are expressed selectively in different tissues. Expression of the identical mature proteins for chorionic somatomammotropin hormones 1 and 2 is upregulated during development, while the ratio of 1 to 2 increases by term. Structural and expression differences provide avenues for developmental regulation and tissue specificity.
Bovine Chorionic somatomammotropin hormone 2 (CSH2) ELISA Kit employs a two-site sandwich ELISA to quantitate CSH2 in samples. An antibody specific for CSH2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCSH2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CSH2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CSH2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Bovine Chorionic somatomammotropin hormone 2 (CSH2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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