Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase (DHDH) gene encodes an enzyme that belongs to the family of dihydrodiol dehydrogenases, which exist in multiple forms in mammalian tissues and are involved in the metabolism of xenobiotics and sugars. These enzymes catalyze the NADP1-linked oxidation of transdihydrodiols of aromatic hydrocarbons to corresponding catechols. This enzyme is a dimeric dihydrodiol dehydrogenase, and it differs from monomeric dihydrodiol dehydrogenases in its high substrate specificity for trans-dihydrodiols of aromatic hydrocarbons in the oxidative direction.
Bovine Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase (DHDH) ELISA Kit employs a two-site sandwich ELISA to quantitate DHDH in samples. An antibody specific for DHDH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDHDH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DHDH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DHDH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Bovine Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase (DHDH) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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