Dihydrofolate reductase (DHFR) converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. This protein acts as an enzyme. It is known to catalyze the following reaction : 5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH
Bovine Dihydrofolate reductase (DHFR) ELISA Kit employs a two-site sandwich ELISA to quantitate DHFR in samples. An antibody specific for DHFR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDHFR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DHFR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DHFR bound in the initial step. The color development is stopped and the intensity of the color is measured.
Bovine Dihydrofolate reductase (DHFR) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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