Malondialdehyde (MDA) is the organic compound with the formula CH2(CHO)2. The structure of this species is more complex than this formula suggests. This reactive species occurs naturally and is a marker for oxidative stress.
Bovine Malondialdehyde (MDA) ELISA Kit employs a two-site sandwich ELISA to quantitate MDA in samples. An antibody specific for MDA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMDA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MDA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MDA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Bovine Malondialdehyde (MDA) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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