NADH-ubiquinone oxidoreductase chain 1 is a protein that in humans is encoded by the mitochondrial gene MT-ND1. The ND1 protein is a subunit of NADH dehydrogenase, which is located in the mitochondrial inner membrane and is the largest of the five complexes of the electron transport chain. Variants of the MT-ND1 gene are associated with mitochondrial encephalomyopathy, lactic acidosis, and stroke-like episodes (MELAS), Leigh's syndrome (LS), Leber's hereditary optic neuropathy (LHON) and increases in adult BMI.
Bovine NADH-ubiquinone oxidoreductase chain 1 (MT-ND1) ELISA Kit employs a two-site sandwich ELISA to quantitate MT-ND1 in samples. An antibody specific for MT-ND1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMT-ND1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MT-ND1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MT-ND1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Bovine NADH-ubiquinone oxidoreductase chain 1 (MT-ND1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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