REAGEN™Aflatoxin M1 ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Aflatoxin M1 in milk,milk powder, starch, vegetable oils, yogurt, buttermilk and soured milk..
The unique features of the kit are:
- No need to extract the sample. The sample can be directly used for the ELISA
- High sensitivity (0.02 ng/g or ppb) and low detection limit in milk(0.05ppb).
- A quick ELISA assay(30 minutes ELISA reaction).
- High reproducibility.
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The plate wells are coated with capture antibodies directed against anti-Aflatoxin M1 antibodies. Aflatoxin M1 standards or sample solution, Aflatoxin M1–HRP conjugate and anti-Aflatoxin M1 antibodies are added. Free Aflatoxin M1 and Aflatoxin M1-HRP Conjugate compete for the Aflatoxin M1 antibody binding sites. At the same time, the anti- Aflatoxin M1 antibodies are also bound by the immobilized capture antibodies. Any unbound Aflatoxin M1-HRP Conjugate is then removed in a washing step. The TMB Substrate is added to the wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
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