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Array CGH Assay
Microarray-based comparative genomic hybridization (array CGH) is a technique used to determine genomic copy number variations. This technology was first developed as a research tool for the investigation of genomic alterations in cancer. Array CGH (comparative genomic hybridization) is now being adopted in the clinical laboratory. It allows for a high-resolution evaluation of DNA copy number alterations associated with chromosome abnormalities. Array CGH is based on the use of differentially labeled test and reference genomic DNA samples that are simultaneously hybridized to DNA targets arrayed on a glass slide or other solid platform. The two samples, a test (or patient) and a reference (or control), are cohybridized onto a solid support (usually a glass microscope slide) on which cloned or synthesized DNA fragments have been immobilized. Microarrays provide some advantages over conventional cytogenetic analysis because they have the potential to detect the majority of microscopic and submicroscopic chromosomal abnormalities. The limitations to BAC array CGH are that regions not represented on an array are not assayed, smaller deletions or duplications are not detected, point mutations will not be uncovered, and balanced chromosomal translocations and inversions cannot be identified. The resolution of the array depends on the size of the cloned DNA targets and the distance between these sequences located on the chromosome. Arrays have been built with a variety of DNA substrates that may include oligonucleotides, cDNAs, or bacterial artificial chromosomes (BACs). This platform is a major contributor to modern cytogenetic diagnostics and provides researchers and clinicians with a powerful tool to improve diagnostic capabilities.
Sample Requirements
Genomic DNA is purified from cells or tissue by several methods, including kits provided by Qiagen (DNeasy) and Gentra (Puregene). At least 1 ug of DNA per sample is required for labeling.
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The leading supplier of molecular diagnostics for customization and production of FISH probes, aGCH assays and upcoming new technologies to be used by Reference labs, Academic labs, Pharma, CRO’s
and Pharma /Biotech companies around the world.
Our companion Diagnostics will allow you to :
-Validate Bio-markers (signature identification and resistance).
-Identify and qualify the aberration at the Chromosomal level of specific population.
-Follow up the result of therapeutic treatment (monitoring, compound profiling, drug response, patient stratification and safety)
-Compare with the result of your current technology.
-Be translated into the initial phase of your diagnostics development toward new FDA filings (pre-clinical through clinical trial development).
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