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- RNAscope® LS Multiplex Fluorescent Assay
- Product Detail
- Company Profile
- Designed as an automated assay on the Leica Biosystems' BOND RX Research Advanced Staining System, this new assay enables single-molecule detection of up to four RNA targets simultaneously. Ideal for co-localization and co-expression studies, the assay and RNAscope® LS 2.5 Probes are available for application in your own lab and via ACD's Pharma Assay Services.
The key technological advantage of this assay is the significant signal boost with simultaneous background noise suppression which is due to advances in ACD’s patented signal amplification technology combined with Perkin Elmer's Tyramide Signal Amplification (TSA™) technology. Furthermore the signal sensitivity is balanced across all three channels. These technological advances enable:
Strong detection of low expressors- Vivid visualization of even very low expressing genes in any of the three probe channels
Compatible with multiple sample types- Detection of signals at low exposure and supports of FFPE samples and fresh frozen samples
Supports dual ISH and IHC protocols- High and stable signals enable sequential detection of proteins by IHC on the same samples. See Dual ISH & IHC for protocols.
Easy to use- No need to match expression level of targets with channel sensitivity
Sharper, crisper signal- Strong balanced signals across all three channels make it easy to generate publication-quality data.
"I was very impressed with the quality of work and quick turn-around of the Pharma Assay Services group. The data generated using RNAscope LS Multiplex kit gave us enough information to proceed further with our study. It allowed us to co-localize our target RNA along with different cell type specific RNA markers to determine in which CNS cell types the target was expressed.”
Curt Mazur
Director, Neuroscience Drug Discovery; Ionis Pharmaceuticals, Inc.
Sample Images
Technology Overview
RNAscope® LS Multiplex Fluorescent Assay allows simultaneous detection of up to four RNA targets within a single sample using up to four target probes and the nuclei can be counter stained and visualized using DAPI fluorescent dye. The four target probes, each designed a different probe channel - C1, C2, C3 or C4, contain several components. The lower part of the double Z probe is designed to hybridize to a specific RNA target. The upper part of the double Z probe, along with the preamplifiers, amplifiers and dyes are assigned to a specific probe channel. Signal detection is performed by Tyramide linked Fluorescein, Cy3 and Cy5 dyes. The sequential assay workflow provides flexibility to visualize any probe in any of the TSA linked fluorophore channels. The combination of ACD patented signal amplification system and the tyramide technology provides a significant boost in the signal while suppressing background noise. This feature makes the assay suitable for use on both FFPE and fresh-frozen samples. It is ideal for co-localization applications and compatible with dual ISH IHC protocols.
Guidelines for Microscope Channel Selection
The RNAscope LS Multiplex Fluorescent Assay supports interrogation of up to 4 target probes, each designed in a separate probe channel C1, C2, C3 and C4, which are visible in the filters associated with the dyes such as FITC, Cy3, Cy5 and Texas Red. The signal amplification in all 4 channels is balanced, removing the necessity of selecting channels based on gene-specific expression level. Customers can freely select channels for their genes. For viewing of 4 plex assay, a multiplexed biomarker imaging system is required, such as Nuance FX Multiplex Biomarker Imaging System, Vectra 3.0 Automated Quantitative Pathology Imaging System or Mantra Quantitative Pathology Workstation from Perkin Elmer.
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Bio-Techne Corporation (NASDAQ: TECH) is a leading developer and manufacturer of high quality purified proteins:notably cytokines and growth factors, antibodies, immunoassays, as well as biologically active small molecule compounds and ACD's in situ hybridization detection products,which are sold to biomedical researchers and clinical research laboratories; these operations constitute the core Biotechnology Division, headquartered in Minneapolis, Minnesota. The Protein Platforms Division manufactures innovative protein analysis tools under the ProteinSimple brand name that greatly automate western blotting and immunoassay practices. The Diagnostics Division manufactures FDA-regulated controls, calibrators, blood gas and clinical chemistry controls and other reagents for OEM customer and clinical customers. Bio-Techne products are integral components of scientific investigations into biological processes and the nature and progress of specific diseases. They aid in drug discovery efforts and provide the means for accurate clinical tests and diagnoses. With thousands of products in its portfolio, Bio-Techne generated approximately $563 million in net sales in fiscal 2017 and has approximately 1,800 employees worldwide. For more information on Bio-Techne and its brands, please visit www.bio-techne.com.
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