REAGEN™ Beta-lactam Strip Test is a qualitative and rapid lateral flow assay designed to detect beta-lactam antibiotic residues in raw commingled cow’s milk. This state-of-the-art test uses a novel beta-lactam binding protein which eliminates the need for a heating block. This test is designed for point-of-care use or reference lab settings.The unique features of the kit are:
l Rapid strip test method – 7 minutes
l Novel binding protein that requires no heating step
l High sensitivity
l High reproducibility
Procedure Overview
The assay uses a competitive colloidal gold based format. The milk sample (200 μL) is added to a clear plastic reaction vessel, and used to resuspend the lyophilized reagents to a uniform pink color in the bottom of the microtiter wells. The milk is incubated briefly (3 min) to allow the beta-lactam binding protein on the gold particles to engage with any beta-lactam antibiotic present in the milk. The test strip is then inserted into the sample well with the arrows pointing downward initiating capillary flow up the strip. Any gold particles that are not complexed with antibiotics present in the milk will bind to the beta-lactam antibiotic imprinted at the Test line (T-line), forming a signal (red line) at that position. If the beta-lactam binding protein on the gold particle has engaged with antibiotic present in the milk sample, the gold particle will flow past the T-line and reach the Control line (C-line). For visual interpretation of the test results, T-line signal intensity that is stronger than the signal at the C-line indicates a negative result. Signal at the T-line which is equal or less intense compared to the C-line indicated the presence of beta-lactam antibiotics in the milk. The greater the reduction in signal intensity at the T-line, the greater the concentration of beta-lactam antibiotics present in the milk.
Sensitivity (Detection Limit)
|
Substance
|
MRL
(ppb)
|
Detection limit (ppb)
|
|
Penicillin G
|
4
|
3-4
|
|
Ampicillin
|
4
|
4-5
|
|
Amoxicillin
|
4
|
4-5
|
|
Cloxacillin
|
30
|
4-8
|
|
Oxacillin
|
30
|
4-8
|
|
Dicloxacillin
|
30
|
5-8
|
|
Nafcillin
|
30
|
15-30
|
|
Ceftiofur
|
100
|
75-100
|
|
Cephapirin
|
60
|
6-15
|
|
Cefazolin
|
50
|
35-50
|
|
Cefoperazone
|
50
|
5-20
|
|
Cephquinome
|
20
|
10-20
|
|
Cephalonium
|
10
|
3-10
|
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