REAGEN™ Nicarbazin ELISA Test Kit enables government agencies, food manufacturers and quality assurance organizations to detect nicarbazin in feed to satisfy customer concerns about food safety.
The unique features of the kit are:
- The extraction of nicarbazin from samples can be finished in 10 - 30 minutes with a high recovery rate of 80 - 105%.
- A quick ELISA assay (less than 1.5 hours regardless of number of samples).
- High reproducibility.
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The nicarbazin antibody has been coated in the plate wells. During the analysis, sample is added along with the Nicarbazin-horseradish peroxidase conjugate. If the nicarbazin residue is present in the sample, it will compete for the nicarbazin antibody, thereby preventing the nicarbazin-HRP from binding to the antibody attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the nicarbazin residue concentration in the sample.
Sensitivity
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Sample Type
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Detection Limit (ng/g or ppb)
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250,000
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