REAGEN™ Flumequine ELISA Test Kit enables government agencies, food manufacturers and quality assurance organizations to detect flumequine in various sample types and to satisfy customer concerns about food safety. REAGEN™ Flumequine ELISA Test Kit provides a unique extraction method, which is accurate, rapid, cost effective and easy to use.
The unique features of the kit are:
The extraction of Flumequine from feed, meat, tissue, serum and urine can be finished with a high recovery rate of 75 - 95%.
A quick ELISA assay (less than 2 hours regardless of number of samples).
High reproducibility.
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The flumequine antibody has been coated in the plate wells. During the analysis, sample is added along with the flumequine-horseradish peroxidase (Flumequine-HRP) conjugate. If the flumequine residue is present in the sample, it will compete for the flumequine antibody, thereby preventing the Flumequine-HRP from binding to the antibody attached to the well. The resulting color intensity,after addition of the HRP substrate (TMB), has an inverse relationship with the flumequine residue concentration in the sample.
Sensitivity (Detection Limit)
|
Sample Type
|
Detection Limit (ng/g or ppb) (Organic Reagent Extraction Method)
|
Detection Limit (ng/g or ppb) (Rapid Method)
|
|
Feed
|
1
|
10
|
|
Meat/Liver/Kidney/Fish/Shrimp
|
0.5
|
10
|
|
Milk
|
1
|
5
|
|
Serum/Urine
|
1
|
5
|
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