Mouse Protein disulfide-isomerase A4 (PDIA4) ELISA Kit,Abbkine_specification/price/image

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Protein Disulfide Isomerase A4is an enzyme in the endoplasmic reticulum in eukaryotes or periplasmic space of prokaryotes that catalyzes the formation and breakage of disulfide bonds between cysteine residues within proteins as they fold. This allows proteins to quickly find the correct arrangement of disulfide bonds in their fully-folded state, and therefore the enzyme acts to catalyze protein folding.The reduced (dithiol) form of PDI is able to catalyse a reduction of mispaired thiol residues of a particular substrate, acting as an isomerase. Therefore, PDI is capable of catalyzing the posttranslational modification disulfide exchange. Such exchange reactions can occur intramolecularly, leading to the rearrangement of disulfide bonds in a single protein.

Mouse Protein disulfide-isomerase A4 (PDIA4) ELISA Kit employs a two-site sandwich ELISA to quantitate PDIA4 in samples. An antibody specific for PDIA4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPDIA4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDIA4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDIA4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Mouse Protein disulfide-isomerase A4 (PDIA4) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/mouse-protein-disulfide-isomerase-a4-pdia4-elisa-kit-kte70777/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.