Pentosidine is a well characterized and easily detected member of this large class of compounds. AGEs are biochemicals formed continuously under normal circumstances, but more rapidly under a variety of stresses, especially oxidative stress and hyperglycemia. They serve as markers of stress and act as toxins themselves. Pentosidine is typical of the class, except that it fluoresces, which allows it to be seen and measured easily. Because it is well characterized, it is often studied to provide new insight into the biochemistry of AGE compounds in general. Derived from ribose, a pentose, pentosidine forms fluorescent cross-links between the arginine and lysine residues in collagen. It is formed in a reaction of the amino acids with the Maillard reaction products of ribose. In patients with Diabetes mellitus type 2, pentosidine correlates with the presence and severity of diabetic complications.
Mouse Pentosidine (PTD) ELISA Kit employs a two-site sandwich ELISA to quantitate PTD in samples. An antibody specific for PTD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTD bound in the initial step. The color development is stopped and the intensity of the color is measured.
Mouse Pentosidine (PTD) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/mouse-pentosidine-ptd-elisa-kit-kte70771/
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