The protein encoded by TAS1R1 is a G protein-coupled receptor and is a component of the heterodimeric amino acid taste receptor T1R1+3. The T1R1+3 receptor responds to L-amino acids but not to D-enantiomers or other compounds. Most amino acids that are perceived as sweet activate T1R1+3, and this activation is strictly dependent on an intact T1R1+3 heterodimer. Multiple transcript variants encoding different isoforms have been found for TAS1R1.
Mouse Taste receptor type 1 member 1 (TAS1R1) ELISA Kit employs a two-site sandwich ELISA to quantitate TAS1R1 in samples. An antibody specific for TAS1R1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTAS1R1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TAS1R1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TAS1R1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Mouse Taste receptor type 1 member 1 (TAS1R1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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