Mouse Brachyury protein (T) ELISA Kit,Abbkine_specification/price/image

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Brachyury appears to have a conserved role in defining the midline of a bilaterian organism,and thus the establishment of the anterior-posterior axis; this function is apparent in chordates and molluscs. Its ancestral role, or at least the role it plays in the Cnidaria, appears to be in defining the blastopore. It also defines the mesoderm during gastrulation.Tissue-culture based techniques have demonstrated one of its roles may be in controlling the velocity of cells as they leave the primitive streak. Transcription of genes required for mesoderm formation and cellular differentiation. Brachyury has also been shown to help establish the cervical vertebral blueprint during fetal development. The number of cervical vertebrae is highly conserved among all mammals; however a spontaneous vertebral and spinal dysplasia (VSD) mutation in this gene has been associated with the development of six or fewer cervical vertebrae instead of the usual seven.

Mouse Brachyury protein (T) ELISA Kit employs a two-site sandwich ELISA to quantitate T in samples. An antibody specific for T has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for T is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of T bound in the initial step. The color development is stopped and the intensity of the color is measured.

Mouse Brachyury protein (T) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/mouse-brachyury-protein-t-elisa-kit-kte70317/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.