ChIP Validated Antibody & Positive Primer Sets
Chromatrap® ChIP-validated Antibodies can now be supplied with the correct matching primer sets to cover all aspects of ChIP validation.
•Reduce time spent searching databases and designing forward and reverse primers
•Purchase superior quality ChIP-validated antibodies with their matching high-quality primers
•Have confidence that the primers are tested with the exact antibody and gene target
The Chromatrap® ChIP Validated Antibody Primer sets include a positive control antibody, a negative control antibody (non immune IgG) to evaluate non-specific binding and species-specific positive control qPCR primers.
ChIP Validated 5mC Antibody with Positive Primer Set
Product Code : 900011
Chromatrap®’s MeDIP Validated 5mC Antibody and Positive Primer Set provides a complete set of tools to assist with a successful ChIP assay. Including: 5mC antibody, control mouse IgG, and a positive primer set. The MeDIP Validated 5mC Antibody and Positive Primer Set is not suitable for use with non-human species.
Background:
5mC (5-Methylcytosine) is a region of DNA that has been epigenetically modified through the addition of a methyl group at the 5th carbon position of cytosine. DNA methylation is a repressive histone modification that is associated with switching off gene expression. This methylation generally occurs at CpG islands and is also associated with genomic imprinting. Aberrant DNA methylation is linked to disease including many cancers.
A mouse IgG is included in this Antibody Primer Set as a negative control for the MeDIP experiment.
The positive primer set included in this Antibody Primer Set recognises an imprinted gene, a source of hypermethylation. It is therefore an appropriate target for the 5mC antibody.
•Applications: MeDIP
•Source: Mouse
• Concentration: 2mg/ml
•Type: Monoclonal
•Size: 50μl
•Purification: Protein G (affinity purified)
•Specificity: Human
•Storage Conditions: -20°C
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