Sample Types Validated:Serum, blood plasma,Saliva, Urine, and other related tissue Liquid.
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
Please read this insert completely prior to using the product. This kit is used to assay Human plasminogen activator inhibitor 1(PAI-1) on the basis of the Biotin double antibody sandwich technology. This Elisa kit takes one-step method with which solutions do not need diluting,because we simplifies the dilute process by our lab techniques. This kit is for research only and is not for use in diagnostic procedures.
Intended Use
This kit is used to assay the plasminogen activator inhibitor 1(PAI-1)in the sample of Human’s serum, blood plasma and other related biological liquid.
Test principle
This kit uses enzyme-linked immune sorbent assay (ELISA) based on the Biotin double antibody sandwich technology to assay the Human plasminogen activator inhibitor 1 (PAI-1). Add plasminogen activator inhibitor 1(PAI-1)to the wells, which are pre-coated with plasminogen activator inhibitor 1(PAI-1)monoclonal antibody and then incubate. After that, add anti PAI-1 antibodies labeled with biotin to unite with streptavidin-HRP, which forms immune complex. Remove unbound enzymes after incubation and washing. Add substrate A and B. Then the solution will turn blue and change into yellow with the effect of acid. The shades of solution and the concentration of Human plasminogen activator inhibitor 1 (PAI-1) are positively correlated.
Materials supplied in the Test Kit
|
1
|
Standard solution(80AU/ml)
|
0.5ml
|
7
|
Chromogen solution A
|
6ml
|
|
2
|
Standard dilution
|
3ml
|
8
|
Chromogen solution B
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6ml
|
|
3
|
Coated ELISA plate
|
12-well * 8 tubes
|
9
|
Stop solution
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6ml
|
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4
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Streptavidin-HRP
|
6ml
|
10
|
instruction
|
1
|
|
5
|
Washing concentrate (30X)
|
20ml
|
11
|
Seal plateme mbrane
|
2
|
|
6
|
Anti PAI-1 antibodies labeled with biotin
|
1ml
|
12
|
Hermetic bag
|
1
|
Materials required but not supplied
1. 37 ℃ incubator 2. Standard Enzyme reader
3. Precision pipettes and Disposable pipette tips 4. Distilled water
5. Disposable tubes for sample dilution 6. Absorbent paper
Contact Rebecca Yan
Skype: span.biotech
Email: rebecca@spanbio.com
Tel: +86(755)89589611
Web:
www.spanbiotech.com
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