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- PrimeTime® qPCR Assays
- Product Detail
- Company Profile
- Primers and probe mixed and delivered in a single tube or plate well
- Online assay design tools provide guaranteed performance
- Available in 5 dye–quencher combinations and 3 reaction scales
- Choose ZEN™ Double-Quenched Probes in your Assay for superior performance compared to traditional dual-labeled probes.
- MIQE Compliant: All primer and probe sequences are provided
- Available in tubes or 96-well plates
- The same primer pairs found in the PrimeTime qPCR 5' Nuclease Assays
- Forward and reverse primers mixed and delivered in a single tube or plate well
- Ideal for use with SYBR® Green, EvaGreen, and other intercalating dyes, where no probe is needed
- Available in tubes, 96-well plates, or Matrix plates
PrimeTime® qPCR 5' Nuclease Assays are probe-based assays—the gold standard for quantitative gene expression studies. Assays are available for various combinations of FAM, HEX, TET, and Cy5®dyes with ZEN™/Iowa Black FQ, TAMRA, and Iowa Black RQ quenchers. Choose from 3 reactions scales—Mini, Standard, and XL—according to the number of samples you plan to analyze.
PrimeTime® qPCR Primer Assays are provided without probe for intercalating dye experiments. Receive 5 nmoles each of forward and reverse primers mixed and delivered in a single tube or plate well, sufficient for 500 reactions (20 µL).
PrimeTime® qPCR 5' Nuclease Assays and PrimeTime® qPCR Primer Assays are available in tubes or 96-well plates. In compliance with MIQE Guidelines, all primer and probe sequences are provided with the order.
PrimeTime® qPCR 5' Nuclease Assays
PrimeTime qPCR 5' Nuclease Assays are offered in three different sizes to meet any qPCR experimental need. In addition, for the Standard and XL sizes, selection of dye–quencher combination and primer-to-probe ratio can be specified to meet unique experimental demands. Assays consist of a forward primer, a reverse primer, and a qPCR probe all delivered in a single tube. Each assay is made to order with estimated shipping in 2–4 days from order receipt. Each oligonucleotide undergoes 100% QC by mass spectrometry and all QC results are provided free of charge to the customer on the IDT website.
PrimeTime® qPCR 5' Nuclease Assays in Tubes
1.See table below for available dye–quencher combinations.2. The primer-to-probe ratio can be specified by the customer (except for PrimeTime Mini).
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| 5' Dye | 3' Quencher | Mini | Standard | XL |
|---|---|---|---|---|
| FAM | ZEN/Iowa Black FQ* | • | • | • |
| FAM | TAMRA | • | • | |
| HEX | ZEN/Iowa Black FQ* | • | • | |
| TET | ZEN/Iowa Black FQ* | • | • | |
| Cy5 | Iowa Black RQ | • | • |
*ZEN™/Iowa Black FQ is a Double-Quenched Probe which provides superior performance to traditional dual-labeled probes. For more information download theZEN Double‑Quenched Probe Overview.
PrimeTime qPCR Primer Assays
PrimeTime® qPCR Primer Assays are predesigned qPCR assays used to detect genes in the human, mouse, and rat transcriptomes. These primer sets are ideal for use with SYBR® Green, EvaGreen®, and other intercalating dyes, where no probe is needed. PrimeTime qPCR Primer Assays are available in Standard Size, premixed and normalized to 5 nmoles per primer, and are shipped dried down. Primer sequences are provided upon order, and the primers ship in 2–3 business days.
PrimeTime® qPCR Primer Assays in Tubes
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About IDT
Integrated DNA Technologies (IDT) is a leader in manufacturing and developing products for the research and diagnostics life science market. IDT serves the areas of academic research, biotechnology, and pharmaceutical development. IDT was founded by Dr Joseph Walder in 1987. Since then, its development has been guided by an uncompromising approach to quality, a belief in the value of good service, and a determination to minimize consumer costs.
Serving over 80,000 life sciences researchers, IDT is widely recognized as the industry leader in custom oligonucleotides due to its capabilities in:
Analytical Sophistication—IDT pioneered the use of high throughput quality control (QC) methods and is the only oligonucleotide manufacturer to offer purity guarantees and 100% QC. Every oligonucleotide is analyzed by mass spectrometry and purified oligonucleotides receive further analysis by CE and HPLC.
Design Engineering—IDT maintains an engineering division dedicated to advancing synthesis, processing technology, and automation. An in-house machine shop provides rapid prototyping and custom part design/control.
Customer Support—IDT received over 100,000 calls last year with an average wait time of only 8 seconds.
Reagent and Input Control—IDT receives all solvents in large bulk containers, runs QC on all incoming materials, and performs bulk reagent formulation and functional QC on all reagents.
IDT has over 700 employees serving our worldwide customer base from headquarters in Coralville, Iowa, and facilities in San Diego, California; Leuven, Belgium; and Singapore.
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