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Postnova: Comparative Analysis of a Complex Monoclonal Antibody

hits:270     Date:05/23/18
Postnova Analytics has published a new poster that compares the suitability of Electrical Asymmetrical Flow Field Flow Fractionation (EAF4) and Size Exclusion Chromatography (SEC) for determining the physicochemical and biophysical attributes of monoclonal antibodies.

In the described work, a reference monoclonal antibody (RM 8671 mAb), from the U.S. National Institute of Standards and Technology (NIST), was used to compare separation, aggregation quantification, and recovery parameters for an EAF4-UV-MALS versus SEC-UV-MALS techniques. The NIST mAb provides a representative test molecule for development of novel technology for therapeutic protein characterization. 

The poster describes how the EAF4 module enabled simultaneous measurement of size and surface charge characteristics (electrophoretic mobility) of antibodies and proteins. Further while no aggregates were detected by SEC, the FFF system showed protein / antibody aggregates represented 10% of the total mass injected. The researchers concluded that the FFF open channel design may allow for better recovery of injected mass than SEC which is particularly important when seeking to quantify aggregates present in small amounts.
Postnova Analytics EAF4 technology uniquely combines the principle of Electrical and Asymmetrical Flow FFF in just one system. In an EAF2000 system - Electrical and Cross Flow Fields are applied simultaneously across the FFF channel enabling separations by size and particle charge based on electrophoretic mobility. Combining these two powerful separation techniques in a single platform opens the door to characterising complex proteins, antibodies and viruses as well as environmental and charged nanoparticles or polymers that have proven intractable to other techniques. 

For a copy of the poster and further information on the EAF2000 system please contact Postnova Analytics on +49-8191-985-6880 / +44-1885-475007 / +1-801-521-2004 / info@postnova.com. 

Founded in 1997, Postnova Analytics is the inventor and leading international supplier of Field-Flow Fractionation (FFF) systems for markets including biopharmaceuticals, polymers, materials, nanotechnology and environmental sciences. Leveraging its unique and patented modular FFF - Light Scattering Platform, Postnova has been able to provide high performance solutions to a wide range of applications. Today the company's growing product portfolio includes Flow FFF, Centrifugal FFF, Thermal FFF, Size Exclusion Chromatography (SEC), MALS and DLS Light Scattering.  Postnova (www.postnova.com) is located and headquartered in Landsberg am Lech (Germany) and has subsidiaries in the USA, UK and Finland. These offices in conjunction with a highly qualified and trained distribution network provides informed local support to customers worldwide.

Worldwide HQ 

Postnova Analytics GmbH
Max-Planck-Straße 14
86899 Landsberg am Lech
Germany 
Tel:  +49-8191-985-6880
Email: info@postnova.com 
Web www.postnova.com
 
UK
 
Postnova Analytics UK Ltd.
Unit 56, The Hop Store
Bishops Frome
WR6 5BT
UK
Tel: +44-1885-475007
 
North America
 
Postnova Analytics Inc
230 South 500 East, S. #120
Salt Lake City, Utah 84102
USA
Tel: +1 801 521 2004
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