Login    Join free Add to favorites    中文
home > news > Tetrodotoxin ELISA Kit for the Direct Detection of Tissue
Industry Updates | New Products | Supplier News | Upcoming Events | business | web
Tetrodotoxin ELISA Kit for the Direct Detection of Tissue

hits:1412     Date:09/18/17
The direct competitive enzyme-linked immunosorbent assay (dcELISA) kit was developed for detecting tetrodotoxin (TTX). The working conditions of the dcELISA kit including the anti-TTX mAb coating concentration, coating method, enzyme-labeled antigen concentration, the antigen diluents, reaction time and temperature were all optimized. The result showed that mAb coating concentration was 3.72 渭g/ml, it was coated at the condition of minimal power treatment of microwave oven for 3 min. The enzyme-labeled antigen concentration was 4.08 渭g/ml. The competitive reaction was under the condition of room temperature 25 掳C for 30 min. The half maximal inhibitory concentration (IC50) of the standard curve was 20.4 ng/ml, detection limit was 1.1 ng/ml, linear range 3.3锝137 ng/ml, the intra-assay CV and inter-assay CV were 6.25% and 7.34% respectively. And recovery rate of TTX ranged from 65.0% to 93.2% with the CV of 9.41锝12.77%. This method is convenient, sensitive and time-saving, hope this dcELISA kit can bring benefits and reference for TTX detection.
Unibiotest develop tetrodotoxin (TTX) ELISA test kit, detect limit is 8 ppb,which can be used to analysis tetrodotoxin (TTX) in batch samples quickly.
Related News>> more

Copyright(C) 2006-2018 Bio-Equip    E-mail:web@bio-equip.cn   娌狪CP澶06040519鍙