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Sequencing Grade Carboxypeptidase B
Origin of place China
Model sequencing grade,sequence analysis,proteomics grade,
Supplier ShangHai YaXin Biotechnology Company
Price 590RMB/mg
Hits 387
Update 2/17/2017
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Sequencing Grade Carboxypeptidase B

Catalog Number:SRCPB01

CAS:9025-24-5

EC:3.4.17.2

Source:Rat carboxypeptidase B, expressed in E. Coli.

 

DESCRIPTION

Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, the pH optimum is 8.0, and pI is 6.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. Recombinant carboxypeptidase B is expressed in E.Coli and purified by high pressure liquid chromatography. There is no trace of other enzyme (such as carboxypeptidase A and chymotrypsin) activity. No protease inhibitors such as PMSF are present in the preparation.

MAIN FEATURES

Source

E. Coli

Purity by HPLC

≥ 95%

Format

Lyophilized

Specific activity

≥ 200 units/mg pro

APPLICATION

Protein structure and sequence analysis, such as hydrolyze basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides.

Antibody quality control.

RECOMMEND USAGE

Prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.5-8.0. The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH 7.0-9.0.

STORAGE INSTRUCTIONS

Recommend recombinant carboxypeptidase B lyophilized should be stored under 2鈩-8鈩 in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20鈩, It is stable within 24 months and no activity lose after 10 times repeated freezing and thawing.

 

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Shanghai Yaxin Biotechnology Co. Ltd. is a biotech company founded in 2008 that focuses on the research and the production of recombinant proteins. Shanghai YaxinBio,located in Shanghai Juke Biotech Park, is ISO 9001 registered company since 2009.


Our main products are Animal Origin Free recombinant carboxypeptidase B and recombinant trypsin, which are of high importance for the production of human recombinant insulin.YaxinBio recombinant carboxypeptidase B and recombinant trypsin have no trace of other contaminating enzyme activities or enzyme inhibitors. They have been successfully used in the production process of recombinant insulin by several companies. Recombinant Trypsin can also be used in the process of cell culture.


Shanghai YaxinBio continuously strives to bring to its worldwide customers the best recombinant protein products. We will continue strengthen our product management and our internal system to meet the demands of biotech companies and scientists in the field of life sciences.

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